Wide-Field Detected Fourier Transform CARS Microscopy

Wide-Field Detected Fourier Transform CARS Microscopy

We present a wide-field imaging implementation of Fourier transform coherent anti-Stokes Raman scattering (wide-field detected FT-CARS) microscopy capable of acquiring high-contrast label-free but chemically specific images over the full vibrational 'fingerprint' region, suitable for a large field of view. Rapid resonant mechanical scanning of the illumination beam coupled with highly sensitive...

Wide-field Fourier transform spectral imaging

Wide-field, high-resolution Fourier ptychographic microscopy

In this article, we report an imaging method, termed Fourier ptychographic microscopy (FPM), which iteratively stitches together a number of variably illuminated, low-resolution intensity images in Fourier space to produce a wide-field, high-resolution complex sample image. By adopting a wavefront correction strategy, the FPM method can also correct for aberrations and digitally extend a micros...

On illumination schemes for wide-field CARS microscopy.

New system for a wide-field CARS microscopy is demonstrated, including two schemes of non-phase-matching illumination. Several advantages including high Stokes pulse energy, pulse-to-pulse stability and inherent synchronization between pump and Stokes pulses were brought by use of methane-filled Raman converter. Spatial resolution of the system with axially symmetric illumination, 0.5 microm, w...

Solid state lasers for wide-field CARS microscopy

We discuss the requirements for laser systems used in Coherent Anti-Stokes Raman Scattering (CARS) microscopy and particularly in its wide-field modification. While such laser parameters as wavelength, spectral width and frequency difference between pump and Stokes beams are similar for all CARS systems, requirements for pulse energy, repetition rate, pulse length and mode structure might be si...